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Mitochondrial D-loop sequence variation among Central Javanese Duck in Indonesia Animal Sciences
Susanti, R; Iswari, Retno Sri; Fibriana, Fidia; Sari, Retno Ika.
 This study was realized to determine the genetic variation of Central Javanese duck based on the D-Loop mtDNA gene. D-loop gene was amplified using PCR technique by specific primer and sequenced using dideoxy termination method with ABI automatic sequencer. ClustalW from MEGA-6.06 software program was employed for multiple alignments of nucleotide sequences. Nucleotide sequences of D-loop gene of mtDMA from the Central Javanese duck were aligned together with other Anas isolates from Genbank using ClustalW of MEGA-6.06 program. The estimation of genetic distance and phylogenetic tree construction were analyzed by Neighbor-Joining method, whereas the calculation of distance matrix was performed using Kimura 2-parameter. Multiple alignments obtained were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetics; Animal sciences mtDNA; D-loop; Central Javanese duck; Genetic variation..
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/34352
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